SINGLE PARTICLE/ TOMOGRAPHY/ MICRO ED

The Simple Origin cryo holders can be loaded with two TEM grids – either standard grids or Autoloader grids for ultra stable cryo imaging. These holders have a long liquid nitrogen (LN2) holding time (10+ hrs), effective cryo protection of samples during cryo transfer and low tip profile. The holder accessories include a workstation with built-in LED and heater and separate units for temperature readout of tip temperature and activated carbon regeneration. The grid clamping is optimized for ease of use under LN2 conditions. The axis symmetric design of the dewar helps provide a stable center of mass of the dewar for a given level of LN2 while tilting the holder during tomography data collection. In addition, the ultra low rate of LN2 evaporation helps to increase the stability and performance of the holder due to the near constant center of mass during a tilt series.

THERMO FISHER SCIENTIFIC/ FEI

MODEL 200 : DUAL AUTOGRID CRYO TRANSFER HOLDER; THERMO FISHER/FEI

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Accepts two Thermo Fisher - Glacios, Arctica and Krios AutoGrids directly for screening in side entry FEI/ThermoFisher TEMs

MODEL 205 : DUAL GRID CRYO TRANSFER HOLDER; THERMO FISHER/FEI

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Accepts two standard 3 mm grids for single particle or tomography data collection in side entry FEI/ThermoFisher TEMs

MODEL 201 : DUAL GRID AND AUTOGRID CRYO TRANSFER HOLDER; THERMO FISHER/FEI

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Accepts one standard 3 mm grid and one Autogrid for single particle or tomography data collection in side entry FEI/ThermoFisher TEMs

JEOL

MODEL 210 : DUAL AUTOGRID CRYO TRANSFER HOLDER; Jeol

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Accepts two Thermo Fisher - Glacios, Arctica and Krios AutoGrids directly for screening in side entry Jeol TEMs

MODEL 215 : DUAL GRID CRYO TRANSFER HOLDER; Jeol

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Accepts two standard 3 mm grids for single particle or tomography data collection in side entry Jeol TEMs

MODEL 211 : DUAL GRID AND AUTOGRID CRYO TRANSFER HOLDER; Jeol

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Accepts one standard 3 mm grid and one Autogrid for single particle or tomography data collection in side entry Jeol TEMs

HITACHI

MODEL 220 : SINGLE AUTOGRID CRYO TRANSFER HOLDER; HITACHI 7800

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Accepts ONE Thermo Fisher - Glacios, Arctica and Krios AutoGrids directly for screening in side entry Hitachi 78XX Series TEMs

MODEL 225 : SINGLE GRID CRYO TRANSFER HOLDER; HITACHI 7800

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Accepts ONE standard 3 mm grid for single particle or tomography data collection in side entry Hitachi 78XX Series TEMs


* All holders are provided with a Workstation, Temperature Controller & a Temperature Monitor

Simple Origin offers a dedicated Pumping Chamber solution (Model 4xx) for up to two TEM holders, which serves not only the purpose of storage, but also allows easy as well as sophisticated cryo holders warmup and regeneration of the dewar internal carbon or zeolite material.

• Consists of DN 63 ISO-K flange (towards turbomolecular pumping station) and DN 25 ISO-KF flange (towards vacuum gauge). Two pumping chambers can be stacked onto a pumping station to allow pumping of up to four holders.
• Transparent housing around the tip areas allows convenient observation.
• Each holder has a dedicated port for pumping the dewar. Each of these pumping ports has a manual vacuum valve.
• Supplied with hose and two blind plugs.
• Compatible with various TEM and cryo holders.
• Can be configured to accept one holder each from two different TEM manufacturers.

THERMO FISHER SCIENTIFIC/ FEI

MODEL 400 : PUMPING CHAMBER ASSEMBLY, TWO HOLDER PORTS - WITH PUMPING SYSTEM, THERMO FISHER/FEI

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MODEL 405 : PUMPING CHAMBER ASSEMBLY, TWO HOLDER PORTS, THERMO FISHER/FEI

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JEOL

MODEL 410 : PUMPING CHAMBER ASSEMBLY, TWO HOLDER PORTS - WITH PUMPING SYSTEM, JEOL

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MODEL 415 : PUMPING CHAMBER ASSEMBLY, TWO HOLDER PORTS, JEOL

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HITACHI

MODEL 420 : PUMPING CHAMBER ASSEMBLY, TWO HOLDER PORTS - WITH PUMPING SYSTEM, HT78XX

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MODEL 425 : PUMPING CHAMBER ASSEMBLY, TWO HOLDER PORTS, HT78XX

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Cryo-microscopy is a powerful technique for the determination of three-dimensional structure of biological molecules at near atomic resolutions. It has been successfully used to create 3D structures of various viruses and proteins to help advance the research pertaining to its prevention and cure. A vital requirement for devices on microscope to achieve imaging biological samples is cryogens like liquid nitrogen. These devices typically have a limited cryogen capacity that can last only up to 2-3 hrs. This limits the window of automatic data acquisition to 2-3 hours and also prevents the use of microscopes during nights. These devices which include “Anti-Contamination Device (ACD)” and specimen holders, typically have a limited cryogen capacity that can last only up to 3-5 hrs. This limits the window of automatic data acquisition to 3-5 hours and also prevents the use of microscopes during nights.

Solution

Cryogen Refilling System is a safe and economical solution that helps cryo-microscopists optimize their research by extending the automatic data acquisition time of the electron microscope!

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