Products

Optimize your research!

Model 200 : DUAL AUTOGRID CRYO TRANSFER HOLDER; THERMO FISHER/FEI

Accepts two Thermo Fisher - Glacios, Arctica and Krios AutoGrids directly for screening in side entry FEI/ThermoFisher TEMs


Model 205 : DUAL GRID CRYO TRANSFER HOLDER; THERMO FISHER/FEI

Accepts two standard 3 mm grids for single particle or tomography data collection in side entry FEI/ThermoFisher TEMs


Model 210 : DUAL AUTOGRID CRYO TRANSFER HOLDER; JEOL

Accepts two Thermo Fisher - Glacios, Arctica and Krios AutoGrids directly for screening in side entry JEOL TEMs


Model 215 : DUAL GRID CRYO TRANSFER HOLDER; JEOL

Accepts two standard 3 mm grids for single particle or tomography data collection in side entry JEOL TEMs


* All holders are provided with a Workstation, Temperature Controller & a Temperature Monitor


Cryo-microscopy is a powerful technique for the determination of three-dimensional structure of biological molecules at near atomic resolutions. It has been successfully used to create 3D structures of various viruses and proteins to help advance the research pertaining to its prevention and cure. A vital requirement for devices on microscope to achieve imaging biological samples is cryogens like liquid nitrogen. These devices typically have a limited cryogen capacity that can last only up to 2-3 hrs. This limits the window of automatic data acquisition to 2-3 hours and also prevents the use of microscopes during nights. These devices which include “Anti-Contamination Device (ACD)” and specimen holders, typically have a limited cryogen capacity that can last only up to 3-5 hrs. This limits the window of automatic data acquisition to 3-5 hours and also prevents the use of microscopes during nights.

Solution

Cryogen Refilling System is a safe and economical solution that helps cryo-microscopists optimize their research by extending the automatic data acquisition time of the electron microscope!

*Patent pending

 


"I am pleased with the auto-filling system you installed last year on our JEM 2200FS microscope. The system runs great and allows us to keep grids in the microscope for more than 4 days without the hassle of filling specimen and ACD dewars manually. It is more reliable than graduate students at night, and at some point we ran it continuously for more than 48 hours, running out of currently maximum of 24 refills."
Sincerely,
Michael Sherman, Ph.D.


Simple Origin, Inc. is the exclusive distributor for TVIPS cameras in North America.


For more than 25 years, TVIPS has manufactured high-performance camera systems for transmission electron microscopy (TEM) with resolutions from 1 to 64 megapixel (MP). Its latest generation of TEM cameras is based on custom-designed, complementary metal oxide semiconductor (CMOS) technology with active pixel sensors. These cameras feature high dynamic range and exceptional acquisition speed. By optimizing the point spread function to the distribution of the scattered electrons in combination with ultra-low noise sensors, single electrons can be clearly detected

TemCam-XF416

  • 16 megapixel camera covering an image area of 63.5 x 63.5 mm²
  • Acquisition speed of up to 50 fps (4k x 4k) or up to 400 fps (512 x 1k) for subareas
  • Ultra Fast Readout
  • Ultra High Dynamic Range of 30000:1
  • Fiber-Optical Coupling
  • Real Time Drift Correction
  • Sensor Cooling
  • Optimized Scintillator

TemCam-F216

  • 2048 x 2048 pixel CMOS sensor
  • Large field of view (32 x 32 mm²); 15.6 µm square pixel
  • 16 bit at 2 x 10 MP readout
  • Readout time at full resolution: 0.6 sec
  • Selectable high-speed readout via rolling shutter
  • Fast readout with up to 8.5 fps (2 k x 1 k pixel)
  • Single electron sensitivity
  • Dynamic range: Typically 10000:1

TemCam-F416R

  • 4096 x 4096 pixel CMOS sensor
  • Large field of view (64 x 64 mm²); 15.6 µm square pixel
  • 16 bit at 4 x 10 MP readout
  • Readout time at full resolution: 1 sec
  • Selectable high-speed readout via rolling shutter
  • Fast readout with up to 8.5 fps (4 k x 1 k pixel)
  • Single electron sensitivity
  • Dynamic range: Typically 10000:1
  • TemCam-F416R version is retractable in the camera chamber (JEOL only)

TemCam-F816 Generation II

  • 8192 x 8192 pixel CMOS sensor
  • Exceptionally large field of view (128 x 128 mm²)
  • Selectable high speed readout via rolling shutter
  • Fast readout with up to 8.5 fps (8 k x 1 k pixel)
  • Single electron sensitivity
  • Optional 1 k x 1 k CCD camera in near-axis (FEI) or on-axis position in the camera chamber (JEOL)

Imaging Software

  • EM-Menu
  • EM-Spectro
  • EM-Tools
  • EM-Conos
  • EM-Scan
  • CLEM






                 

Frequently asked questions

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Q1: What is the maximum tilt range of the Dual Grid/Autogrid Cryo Transfer Holder?
Answer: The holder has a tip thickness of 1.5mm. It is designed for a alpha tilt of +/- 70 degrees. Please note that the maximum tilt is mainly determined by the pole pieces of your TEM. Please consult with your TEM manufacturer (providing the 1.5mm value for our tip height) and/or Simple Origin for specific information.

Q2: How often do we need to pump the Dual Grid/Autogrid Cryo Transfer Holder dewar and how often should we regenerate the zeolite? Which pumping system should be used and which vacuum level should be achieved?
Answer: Please use an oil free pumping system that includes a turbo pump. A desired dewar vacuum is in the low 10-6 torr range. It is usually recommended to pump the dewar overnight before using it. The zeolite regeneration is required less often and should be typically performed once per week to once per month (in case the dewar is vented accidentally, longer pumping and multiple zeolite regeneration is required).
A clear indication of required pumping and/or zeolite regeneration is noticeable condensation on the outer dewar surface.

Q3: What are the drift and resolution specifications of the Dual Grid/Autogrid Cryo Transfer Holder?
Answer: Our Dual Grid/Autogrid Cryo Transfer Holders are designed to achieve steady state drift values below 3 nm/min with a resolution better than 2 Å during regular operations.
It is important to note that drift and resolutions are dependent on various parameters such as TEM performance, objective lens, sample, and camera conditions.
The overall baseline reference for the achievable values is usually set by the performance of the TEMs standard single tilt holder at room temperature.

Q4: What is the liquid nitrogen holding time of Dual Grid/Autogrid Cryo Transfer Holder in the TEM?
Answer: The Dual Grid/Autogrid Cryo Transfer Holder has a steady state holding time of ~10 hrs. Our cryo holders work seamlessly with the Cryogen Refilling System that allows for extended automatic data acquisition time of up to ~48 hrs.

Q5: Is the TEM grid (or Autogrid) emerged in liquid nitrogen or surrounded by cold nitrogen gas within the workstation?
Answer: The workstation is typically filled with liquid Nitrogen upto the holder Tip. The Tip is surrounded by cold nitrogen gas during sample loading.

Q6: How do you measure the sample temperature ?
Answer: In the Dual Grid/Autogrid Cryo Transfer Holder we use a calibrated silicon diode that is located ~ 25 mm away from the sample position.

Q7: What transfer temperatures do you achieve while using the Dual Grid/Autogrid Cryo Transfer Holder(from the workstation to the TEM and back) ?
Answer: We usually achieve transfer temperature < -175°C. This is well sufficient to prevent recrystallization of amorphous ice.

Q8: What is the base temperature of Dual Grid/Autogrid Cryo Transfer Holder ?
Answer: Typical steady state tip temperatures are below -180°C.

Q9: How long does it take to cool down the Dual Grid/Autogrid Cryo Transfer Holder in the workstation ?
Answer: It takes 15- 30 minutes to achieve temperatures below -175°C independent of tip extraction. Allowing enough time for that cool down process is recommended to maintain good sample quality.

Q10: How should we store the Dual Grid/Autogrid Cryo Transfer Holder when not in use?
Answer: It is recommended to store the holder under vacuum on the pumping station

Q11: How should we warm up the workstation and cryo holder after usage ?
Answer: To warm up the workstation and the Dual Grid/Autogrid Cryo Transfer Holder it is recommended to
  • Pour out the liquid Nitrogen from the workstation and the holder dewar
  • Switch on the workstation built in heater. (A hair dryer can be useful to speed up the process)
  • Connect the controller to the holder and begin the warm up cycle.
  • Flowing dry nitrogen gas in the workstation and holder dewar helps keep the moisture out/li>
  • If the holder is on the pumping station it can be positioned upside down with the dewar opening facing downwards
Before using the workstation/holder again, please make sure that they are completely dry.

Q12: How do we clean the holder?
Answer: Using appropriate plasma cleaners enables the removal of hydrocarbons from the holder tip.

Q1: What TEM’s are compatible with the Refilling System?
Answer:
  • The Cryogen Refilling system has been successfully installed on FEI TF20, TF30, T12, Talos F200C & Titan TEM’s and JEM 2200 TEM.
  • It is also compatible with FEI CM200 & CM300 TEM’s and JEM 2100 & JEM1400 TEM’s.

Q2: How big is the refilling system dewar?
Answer: The refilling system dewar is 10 liters. You can get up to 48 hrs of unattended data collection using the system.

Q3: Is there liquid nitrogen level sensor in the Dewar?
Answer: We have a patented sensor for the cryo specimen holder dewar as well as the ACD (cryo trap) dewar. This sensor detects the liquid nitrogen level during refills and closes the valves to prevent liquid nitrogen from overflowing during refills.

Q4: Can the sensor be customized for any Cryo Sample Holder?
Answer: Yes, we make the sensor for all the commercially available cryo sample holders.

Q5: Can the system be used to fill the ACD (cold trap) dewar?
Answer: Yes, the refilling system can fill both the holder and the ACD dewar. This selection can be made in the user interface.The GUI has an option for the user to select each dewar independently for refills

Q6: Can the operation be integrated with third party data collection software?
Answer: Presently, the system is integrated with TVIPS data collection software EM-Menu. We are working on integrating it with Serial-EM in the near future.

Q7: Can we control the system remotely?
Answer: The system communicates with the camera/TEM PC and continuously writes the system parameters to the windows registry. These can be used to monitor and start/stop the system remotely.

Q8: How long does it take to fill the cryo holder dewar?
Answer: Users usually refill the Gatan 626 holder every 3 hrs and it takes about a minute to fill it.

Q9: What are the safety features built in the system?
Answer: The system is designed by keeping in mind the complete data collection process. Some of the safety features include:
  • The system can detect if the holder is present and will stop the refill operation if there is a missing holder
  • The system will not refill in case of communication error from the sensor
  • The system cryo valves will close in case of power failure or loss of control gas pressure

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    At Simple Origin, our team is motivated to solve engineering challenges using innovative solutions. Our mission is to collaborate with scientists to identify and develop next generation research instruments.

  • Pushkarraj Deshmukh

    Founder & President

    p.deshmukh@simpleorigin.us


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